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γ 32p atp  (New England Biolabs)


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Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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    Structured Review

    New England Biolabs γ 32p atp
    γ 32p Atp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B3+32p+atp/T4+Polynucleotide+Kinase/pmc13173289-68-5-10
    Average 99 stars, based on 11327 article reviews
    γ 32p atp - by Bioz Stars, 2026-11
    99/100 stars

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    other:

    Article Title: Phage-encoded small RNA hijacks host replication machinery to support the phage lytic cycle.
    Article Snippet: PCR products were ligated according to the NEBuilder assembly master mix protocol (New England Biolabs).

    Labeling:

    Article Title: YBX1 downregulation links to impaired spermatogonial stem cells function potentially through inhibiting miRNAs production
    Article Snippet: The RNA was then electrotransferred onto positively charged nylon 537 membranes (Roche Diagnostics) using a semi-dry transfer system (Bio-Rad) and immobilized by UV 538 crosslinking at 125 mJ/cm2. .. DNA oligonucleotides complementary to the target miRNAs were 5′-end 539 labeled with [γ-32P]ATP using T4 polynucleotide kinase (NEB). .. Hybridization was carried out at 58 °C in 540 ExpressHybTM Hybridization Solution (Takara).

    Article Title: MicroRNA-199a-3p suppresses non-small cell lung cancer progression by targeting FTO to enhance m6A-mediated downregulation of MZF1 and its transcriptional activation of CLDND1
    Article Snippet: .. The precipitated RNA was labeled with (γ-32P)-ATP using T4 polynucleotide kinase (New England Biolabs) for 30 min at 37°C, resolved by 10% SDS-PAGE, transferred to nitrocellulose, and visualized by autoradiography. .. RNA concentration and purity were measured with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific).

    Article Title: Phylogenetic and functional characterization of Asgard primases.
    Article Snippet: The 21 labeled template was prepared by annealing a labeled primer (D25 or R25, Table S1) with 22 M13mp18 ssDNA (New England Biolabs, USA) at 1:1.25 molar ratio. .. D25 and R25 were 23 AC CE PT ED M AN US CR IP T D ow nloaded from https://academ ic.oup.com /m be/advance-article/doi/10.1093/m olbev/m saf330/8403469 by guest on 30 D ecem ber 2025 labeled at the 5’ terminus using [γ-32P]ATP and T4 polynucleotide kinase (New England Biolabs, 1 USA). ..

    Incubation:

    Article Title: JULGI coordinates vascular development and leaf patterning through non-cell-autonomous regulation of miR165/166.
    Article Snippet: .. RNA probes were labelled with [γ-32P]-ATP (10 mCi ml−1) by incubation with T4 polynucleotide kinase (New England Biolabs) for 30 minutes at 37°C. .. The unlabeled radionucleotides were removed using an Illustra MicroSpin G25 column (Amersham).

    Article Title: Ribosomal protein control of hematopoietic stem cell transformation through regulation of metabolism
    Article Snippet: .. 15pmol of each RNA oligo was incubated with 40μCi of γ-32P-ATP and 10U T4 polynucleotide kinase (NEB, M0201S) in 1x T4 polynucleotide kinase buffer (NEB) for 40 min at 37°C. .. Free ATP was removed using a NucAway spin column (ThermoFisher, AM10070).

    Autoradiography:

    Article Title: MicroRNA-199a-3p suppresses non-small cell lung cancer progression by targeting FTO to enhance m6A-mediated downregulation of MZF1 and its transcriptional activation of CLDND1
    Article Snippet: .. The precipitated RNA was labeled with (γ-32P)-ATP using T4 polynucleotide kinase (New England Biolabs) for 30 min at 37°C, resolved by 10% SDS-PAGE, transferred to nitrocellulose, and visualized by autoradiography. .. RNA concentration and purity were measured with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific).



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